Category:Food Science’

Science on a Stick: Rock Candy

 - by KitchenPantryScientist

Got sugar? You can grow big, edible sugar crystals, commonly called “rock candy,” in your own kitchen.  We thought they’d make a great science experiment to demonstrate at the Minnesota State Fair, where foods on a stick hold sway.

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Like bricks in a wall, crystals are solids formed by repeating patterns of molecules. Instead of mortar, the atoms and molecules are connected by atomic bonds.

They can be big or small, but crystals made from the same atoms or molecules always form the same shape. Table sugar, or sucrose, is made up of a molecule composed of two sugars, glucose and fructose.  The crystals formed by sucrose are hexagonal (six-sided) prisms, slanted at the ends.

To make rock candy on a stick, you’ll need: 5 cups white granulated sugar, 2 cups water, cake pop sticks or wooden skewers, and food coloring

Seed Crystals

  1. Dip one end of cake-pop sticks or wooden skewers in water and then roll them in granulated white sugar. The sugar should cover 2-3 inches of the stick. Let them dry completely. These are the seeds for the sugar crystal growth.
  2. Boil 2 cups water and 5 cups sugar until sugar is dissolved as much as possible. It should look like syrup. This is your supersaturated sugar solution.
  3. Let syrup sit until it is no longer hot and pour into glass containers. Add food coloring and stir.
  4. When colored syrup is completely cool, set the sugary end of the sugar-seeded cake pops or skewers into the syrup and let them sit for about a week.

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5. Gently move the sticks around occasionally, so they don’t stick to the crystals in the bottom of the glass. If the glass container gets too full of crystals, pour the syrup into a new container and move your stick into the cleaner syrup to grow more crystals. When the rock candy is done, drain the excess syrup and let them dry. Enjoy!

sugar crystals

The science behind the candy?  A supersaturated solution is one that is forced to hold more atoms in water or another solute than it normally would. Supersaturated solutions can be made using heat or pressure.  Crystals start to form when a supersaturated solutions encounters a “seed” atom or molecule, causing the other atoms to come out of the solution and attach to the seed. In this case, the seed molecules were the sucrose molecules we dried onto the sticks.

 

The Science of Hard-Boiled Eggs

 - by KitchenPantryScientist

You can’t judge an egg by its shell, but you can use science to figure out whether or not it’s fresh.

Imagine an egg. It can be white or brown, since they’re identical except for shell color. There are two membranes inside an eggshell, separating it from the inside of the egg and helping to keep it safe from microbial invaders.

Under the membranes is the egg white, made up of proteins and water, and the yolk, which also contains fat and is enclosed in a sac. Tiny rope-like structures anchor the yolk between either end of the egg. The egg white contains a substance called lysosyme, which is a potent antibacterial. Eleven percent of an egg’s weight is made up by shell, 58% by white and 31% by yolk.

When a hen first lays an egg, the raw egg white contains carbon dioxide, making it look cloudy, and the proteins in the egg white are freshly folded into their correct protein shapes, so it will hold a nice shape in a pan. However, egg shells contain thousands of tiny pores, some big enough to see with the naked eye, and as an egg sits, it changes.

The contents begin to slowly shrink, and a small air pocket forms between the two membranes, usually at the large end. The egg’s pH, about 7.6 when first laid, rises as the egg ages and loses carbon dioxide. In just a few days, the pH may reach 9.7, causing the egg white to look clear and spread out more in a pan when the egg is broken.

The nicer shape and centered yolk of fresh eggs is why they’re recommended for frying. But why are older eggs better for boiling, and why does the yolk turn green sometimes?

Fresh eggs are harder to peel. When you boil an egg, it cooks from the outside to the inside, and its proteins become unfolded, or denatured. The denatured proteins are more likely to stick to the membranes on the eggshell of a fresh egg because the pH is lower. According to “FOODS, A Scientific Approach” by Charley and Weaver, eggs are easier to peel if their pH is greater than 8.7. In other words, old eggs that have lost carbon dioxide have a higher pH (are less acidic) and are easier to peel.

Sometimes, when you boil eggs, you see a greenish/gray/blue layer on the outside of the yolk. It’s the harmless product of a chemical reaction between the iron in the egg yolk and sulfer-containing proteins in the white. You can try to avoid it by using fresh eggs, using hot (not boiling water) to cook the eggs, by plunging eggs into ice water immediately after cooking, and by promptly removing the shells.

If you’ve heard of candling eggs, it involves shining a strong light through a raw egg to look at yolk position, air sac size and white clarity. You can also tell that an egg is older if it floats in water, due to the enlarged air sac.

Ideally, to cook perfect hard boiled (large) eggs, you put them in cold water, bring the water to a boil, remove the heat and let the pan stand with the lid on for 17 minutes before removing the eggs and plunging them into cold water. Alternately, boil large eggs for eleven minutes and put them in ice water to stop the cooking. For perfect eggs, prick the large end of your eggs with a pin to release the air in the air sac.

Kitchen Science

 - by KitchenPantryScientist

“Are you a good cook?” was the first thing Dr. Tsneo Suzuki asked when I sat down in the office next to his cancer research lab at the University of Kansas. I stared at the picture of his wife, who I later learned had passed away from breast cancer, and wondered whether I should be offended.

After all, I was in my twenties and had five years of molecular biology experience under my belt. But I understood why he asked the question. Once you figure out how to test a hypothesis, most science experiments involve following recipes, which scientists call protocols. Generally, if you can read directions and mix things together in the correct order, in the right proportions, you can do things like amplify DNA and clone genes into bacteria.

So I truthfully answered “Yes, I’m a pretty good cook,” and got the job.

Food preparation is like a science experiment. If you can follow a recipe, you should get something close to what you set out to make, because often the ingredients will interact with each other to make something new. This is the very definition of a chemical reaction. Everything you cook with, from water to baking soda, is just a collection of molecules.

Here’s a collection of some food science experiments on my website. Since I love to cook, I hope to add more in the future! Leave a comment if you have other favorite kitchen science experiments, and I’ll try to add them to the list.

Testing Foods for Starch- Add a drop of iodine and watch for color change to detect starch.

Crock Pot Microbiology: Making yogurt from scratch is a delicious experiment

Yeast Experiment: Pyramids, Pasteur and Plastic Baggies- Grow yeast in a plastic bag to see how they make bread rise.

Emulsions: Mayonnaise and Vinaigrette- Mix the un-mixable using surfactants.

Curds and Whey: Make glue and plastic from milk and vinegar.

Gluten Ball- Explore the protein that makes bread chewy.

Red Cabbage Juice CO2 experiment- Use the pH-sensitive pigment in red cabbage to illustrate how CO2 can acidify liquids (and why soda is bad for your teeth.)

Homemade Petri Plates: test surfaces around your kitchen and house for microbes.  Use to test fingers before washing, after washing with water alone, after washing with soap, and after using hand sanitizer.

So remember, cooking can make you a better scientist, and doing science can make you a better cook.

 

 

Crock Pot Microbiology: Yogurt

 - by KitchenPantryScientist

Microbes are always fighting for space.

Bacteria and fungi try to outnumber other tiny competitors using chemical warfare, among other things.  That’s why many antibiotics (which kill certain bacteria) are actually produced by other bacteria. One reason foods like yogurt and cheese, which are made by beneficial bacteria like Lactobacillus acidopholis, don’t easily spoil is that these bacteria  can turn milk sugars into lactic acid. This makes their environment toxic to some of their competitors, like pathogenic bacteria.  Luckily, we humans aren’t harmed by lactic acid and can enjoy its tangy flavor.

To grow bacteria in labs, scientists have to take care of them the way you’d take care of a pet.  You have to give them the type of food they like, the right amount of oxygen and moisture, and keep them at their optimal temperature.

The same principles apply to growing the bacteria that make yogurt.  You prepare the bacteria’s food by heating some milk and letting it cool to a temperature that the bacteria can tolerate. Then, you add the bacteria and let them grow for about eight hours.  During that time, the bacteria will happily divide, multiply and eat milk sugar. In the process, they’ll produce lots of lactic acid which changes the way the proteins and fats in the milk interact, forming a more solid food product.

We made yogurt in our crock pot, which turned out to be a lovely bacterial incubator. The end product was a little runny, but putting it through cheese cloth (or a coffee filter in a plastic bag with the tip cut off) gives you thicker yogurt.  It is delicious!  Here’s how we made it, thanks to directions from Stephanie O’Dea:

Ingredients: 8 cups (half-gallon) of whole milk , 1/2 cup grocery store yogurt  (must contain live/active culture), thick bath towel, slow cooker

Turn crock pot on to low. Add an entire half gallon of milk. Cover and cook  for 2 hours and 30 minute. Unplug your crock pot, but leave the cover on. Let it sit for 3 hours so your bacteria will not be overheated when you add them.
After 3 hours, put 2 cups of your warm milk  in a bowl. Whisk in 1/2 cup of the live/active culture yogurt. Dump the bowl contents back into the crock pot and stir well. Wrap a heavy bath towel all the way around the unplugged crock pot as insulation and let your bacteria grow for 4-8 hours or until thickened.  Refrigerate and enjoy with fruit, honey, or granola.  As I mentioned, you can strain the yogurt if you prefer a thicker consistency, and your homemade yogurt will make a great starter culture for the next batch!

If you don’t have cheesecloth, you can strain your yogurt through a coffee filter in a plastic bag with a corner cut off.

Happy kitchen microbiology!

Emulsions: Mayonnaise and Vinaigrette

 - by KitchenPantryScientist

“When I wasn’t at school, I was experimenting at home, and became a bit of a Mad Scientist. I did hours of research on mayonnaise, for instance, and although no one seemed to care about it, I thought it was utterly fascinating. When the weather turned cold, the mayo suddenly became a terrible struggle, because the emulsion kept separating, and it wouldn’t behave when there was a change in the olive oil or the room temperature. I finally got the upper hand by going back to the beginning of the process, studying each step scientifically, and writing it all down. By the end of my research, I believe, I had written more on the subject of mayonnaise than anyone in history. I made so much mayonnaise that Paul and I could hardly bear to eat it anymore, and I took to dumping my test batches down the toilet. What a shame. But in this way I had finally discovered a foolproof recipe, which was a glory.” Julia Child, from My Life in France

Julia’s secret for fool-proof mayo? Beat the mixture over a bowl of hot water to get the oil and eggs to form an emulsion, which is a mixture of two thing which are normally immiscible, like water and oil. In an emulsion, a bunch of one type of molecule will actually surround individuals or small groups of  the other type of molecule (think ring-around the rosy with one or two people in the middle who would rather not be there.)  When you’re trying to make an emulsion, it also helps to add a mediator called a surfactant to get between and interact with the immiscible molecules to stabilize the mixture. In a vinaigrette prepared using oil, mustard and vinegar, the proteins in the mustard act as surfactants.  In mayonnaise, adding a little water to the eggs before adding the oil helps make some of the proteins in the eggs more available to act as surfactants.  Of course, adding a little mustard helps too and tastes great!

You can tell when an emulsion begins to form, because your mayo or vinaigrette will start to look lighter-colored and thicker as the molecules are rearranged and reflect light differently!

Remember that a bacteria called Salmonella enteriditis can lurk in raw eggs and make you sick, so it’s better to use pasteurized eggs for recipes like mayonnaise, where you don’t cook the eggs.

Here’s the New York Times recipe we used to make mayonnaise:

  • 1 large egg yolk, at room temperature
  • 2 teaspoons lemon juice
  • 1 teaspoon Dijon mustard
  • 1/4 teaspoon kosher salt
  • 1 teaspoon cold water
  • 3/4 cup neutral oil such as safflower or canola
In a medium bowl, whisk together the egg yolk, lemon juice, mustard, salt and 1 teaspoon cold water until frothy. Whisking constantly, slowly dribble in the oil until mayonnaise is thick and oil is incorporated. When the mayonnaise emulsifies and starts to thicken, you can add the oil in a thin stream, instead of drop by drop.

Like salad? To make delicious vinaigrette, whisk together 1 Tbs. vinegar and 1 Tbs. mustard (the surfactant.) Now, add 3 Tbs. oil, drop-by-drop until you see your emulsion form!

Try some variations on these kitchen experiments. Does it work better to use a cold egg, room temperature egg, or warm egg?  What happens if you try to make mayo by setting your mixing bowl in a bowl of ICE water? Do you get an emulsion?

Can you see the difference between batches of vinaigrette? One was whipped over a bowl of ice water and the other over warm water.

 

As Julia Child would say, “Bon Appetit!”

Thanksgiving Science Experiment: testing foods for starch

 - by KitchenPantryScientist

Ever wonder how much starch is in your Thanksgiving dinner?  Click here for a fun experiment that lets you test your favorite foods for starch using iodine from the medicine cabinet.

Remember to supervise small children if you do this experiment, since iodine should not be ingested! Happy Thanksgiving!

Gluten Ball

 - by KitchenPantryScientist

Gluten is a hot topic these days, but most people aren’t really sure what it is.

Gluten is a protein found in wheat, rye and barley. It makes bread chewy and helps hold the bubbles that yeast makes in dough so that it can rise. (Here’s a fun yeast experiment!) Although some people have a disorder called celiac disease which impedes them from digesting gluten properly, most people can eat it without any problem.

You can easily extract a ball of gluten from flour using nothing but your hands and cold water, to see for yourself what this stretchy grain protein looks like. Just add a cup of water to a cup of flour, mix it together and knead it for about 5 minutes. (Add more flour if it’s too sticky to handle.)

Now, put the dough under COLD water and start “washing” out the carbohydrates so that mostly the gluten remains. Your hands will freeze, so you may need to take a break. If you keep going until the water coming off the dough is mostly clear, you’ll be left with a gluten ball.

Some types of flour have more gluten than others, and sometimes extra gluten is added to pizza dough to make it chewier! Try this with several types of flour to see what has the most gluten (or no gluten.)

Ocean Acidification Experiment/ CO2 Breath Test

 - by KitchenPantryScientist

Last week, I wrote about Carbon, Bananas, Coal and You and promised to try to come up with a safe, easy way to see the carbon dioxide in your breath, so here it is! If you prefer a video, click here to see a quick demo of the experiment on Kare 11.

Once again, the star of the show will be red cabbage juice, a safe, natural, easy-to-make acid/base indicator and the same one you can use to make magic potion and red cabbage litmus paper. The trick is to use a very small volume of cabbage juice, since it’s not a very sensitive acid indicator.

You’ll need red cabbage, drinking straws, and very small cups (the ones you measure kids’ medicine with work well) or test tubes.  Chop a head of red cabbage, boil it for 5-10 minutes, and collect the juice. It will be purple and turns blue when exposed to a base or pink when exposed to an acid.

Then, pour a very small volume- a teaspoon or two (5 to 10 ml)- of the (cooled) juice into two small cups. Take a straw, put it all the way against the bottom of one cup and blow through the straw repeatedly for a few minutes until you see the cabbage juice turn noticeably pinker than the juice in the control cup. It may take several minutes to see a difference, so be patient! Test tubes are less messy since the juice can’t splatter so much.

What happens? The carbon dioxide in your breath combines with the water in the cabbage juice to form carbonic acid, causing the pH of the solution to drop and the cabbage juice to turn pink.

Why is this interesting? About a quarter of the carbon dioxide released by activities like burning fossil fuels and burning down rainforests is absorbed by our world’s oceans. This results in the ocean water becoming more acidic, like the cabbage juice in the experiment, and can have an effect on sea life, like coral. To learn more about ocean acidification and the chemistry of ocean acidification, check out NOAA’s amazing website.

You can explore the same concept (and see why carbonated drinks are hard on your teeth) by pouring uncarbonated water into one cup of cabbage juice and carbonated water into another.  If you can, choose water from the same source, so you know the only difference is the carbon dioxide that’s been added to make it fizzy! Or, you could use dry ice to add carbon dioxide bubbles to water and test it before and after you add bubbles!

What happens if you add yeast to cabbage juice and let it grow for a while?

You can use your leftover cabbage juice to make red cabbage litmus paper and then for a “magic potion” experiment.

Food Science: Red Cabbage Litmus Paper

 - by KitchenPantryScientist

This is a great science project and results in beautifully colored paper that can be dried and used for art projects like collages.

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All you’ll need is a head of red cabbage and some paper towels or white coffee filters.  Alternately, you can just use the juice from canned red cabbage.  I’d recommend wearing an old tee shirt or a home-made lab coat for this project, since I’m guessing that cabbage juice stains.   To make a lab coat, just have kids write their name in permanent marker on the pocket of an old button-down shirt. 

Chop half a head of red cabbage into small pieces and add it to a pan with about a cup of water.  Boil the cabbage uncovered for about 15 minutes, stirring occasionally, let it cool, and strain the juice into a jar or bowl.  (Save the cooked cabbage for your favorite recipe and make cole slaw with the other half!)

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If you want to avoid the stove, chop half a head of red cabbage and blend it with about 3 cups of water. Strain the liquid through a colander and then through a coffee filter in a plastic bag with one corner cut off. Blended cabbage juice makes longer-lasting bubbles and turns a slightly brighter shade of blue!

Cut the paper towels or coffee filters into strips about an inch wide and a few inches long and soak them in the cabbage juice for about a minute.  Remove them and let them dry on something that won’t stain.  I blotted them a little to speed up the drying process.  You might even try using a blow dryer!

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When dry, your litmus paper will be ready to use for testing acidity.  Your can dip the paper into orange juice, soapy water, lemon juice, baking soda in water, baking powder in water, vinegar, and anything else they want to test.  The paper will turn red-pink in acids and blue or green in bases. 

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Everything in our world is made of very tiny pieces called atoms.  Atoms are so small that if you blow up a balloon, it will contain about a hundred billion billion atoms of the gases that make up air.  Atoms are often bonded to other atoms to form a group of linked atoms called a molecule.  A water molecule, for example, has two hydrogen atoms and one oxygen atom, bonded together.

Acids usually dissolve in water to form free-floating hydrogen atoms.  Bases are the opposite and take up free hydrogen atoms.  The molecules in the cabbage juice litmus paper change when exposed to an acid or base, making the paper change color.

How Safe is Your Milk?

 - by KitchenPantryScientist

For years the F.D.A. has been threatening to do more rigorous testing of  milk supplies for antibiotics.  Now they’re going through with it, and many in the dairy industry are furious.

According to an article I read in the New York Times, inspectors have found both illegal levels of antibiotics, and types of antibiotics not regularly tested for, in older dairy cows that have been sent to slaughter.  Anyone who doesn’t want their children getting an extra dose of drugs with their glass of milk should find this disturbing.

The fact that one large dairy COOP in the NorthEast told members to dump milk tested by the FDA in order to avoid recalls indicates that many in the industry are worried about what inspectors will find once they begin testing.

Large-scale agriculture is rife with antibiotics that are fed to beef cows to make them grow faster.  What do consumers have to do to stop the overuse of these drugs in our food supply?

I plan to keep buying organic milk, hoping the tighter regulation and safer farming practices will keep my food pristine.

And please, test away, F.D.A.  It’s your job to protect consumers.  Don’t be afraid to do it.